Biodegradable Potential of Bacillus amyloliquefaciens and Bacillus safensis Using Low Density Polyethylene Thermoplastic (LDPE) Substrate

An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens

Low-density polyethylene (LDPE) is a major cause of persistent and long-term environmental pollution. In this paper, two bacterial isolates Bacillus amyloliquefaciens (BSM-1) and Bacillus amyloliquefaciens (BSM-2) were isolated from municipal solid soil and used for polymer degradation studies. The microbial degradation LDPE was analyzed by dry weight reduction of LDPE film, change in pH of cul...

Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis.

Eight strains of highly amylolytic, sporeforming bacilli (hereafter referred to as Bacillus amyloliquefaciens) were compared with respect to their taxonomic relationship to B. subtilis. The physiological-biochemical properties of these two groups of organisms showed that B. amyloliquefaciens differed from B. subtilis by their ability to grow in 10% NaCl, characteristic growth on potato plugs, i...

Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. Bacillus pumillus and Bacillus safensis, are species of biotechnological and pharmaceutical significance, difficult to differentiate by con...

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

Transformation in Bacillus amyloliquefaciens.

The methodology and some of the requirements for the deoxyribonucleic acid-mediated transformation of an arginine auxotroph of Bacillus amyloliquefaciens to prototrophy are described.